Coding

Part:BBa_K2762002:Design

Designed by: Ting-Shou Liu   Group: iGEM18_NCKU_Tainan   (2018-09-29)


Small subunit of the ribulose-bisphosphate carboxylase/oxygenase rbcS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcS sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

Codon oprimized Synechococcus elongatus PCC7002.

References